Transgenic poplar(Populus alba×P.glandulosa cv‘84k’) plants with the coleopterous insect resistant genes (BtCry3A and OC-I)were obtained. The transgenic nature of these plants was confirmed by PCR amplification and dot hybridization. The transgenic poplar’s toxicity towards the Anoplophora glabripennis larvae was assessed on two year-old selected plants in laboratory conditions. The results indicated that the transgenic lines BOGA-38 and BOGA-39 were deleterious for A. glabripennis larvae, and BOGA-5、BOGA-31、BOGA-38、BOGA-39 could inhabit the growth of A. glabripennis larvae. BOGA-39 was the most toxic one among the transgenic lines, with (41.18 %) corected mortality rate and 78.90 % growth inhabit rate for the larvae.

目的 研究Esteya vermicola真菌适应的pH范围，并探讨松树成分对其生长的影响，以期为E. vermicola菌在松树体内的定植和林业重大病害松材线虫野外的生物防治提供理论支撑。 方法 选取E. vermicolaCBS115803（EV115803）和E. vermicolaCNU120806（EV120806）为试验对象，测试EV真菌适应的pH范围，并以35 a黑松不同器官组织配制培养基，探究EV菌的生长情况，观测各菌株生长速率、菌体量和产孢量。 结果 EV菌适应的pH值范围为5～13，菌体量在酸性条件下产生更多的气生菌丝。PDA中加入松树成分对EV菌有一定的刺激生长作用。在以松针、松树皮、松枝条为唯一营养来源时，EV菌生长茂盛。以木质部锯末为单一营养源时，EV菌生长缓慢。 结论 EV菌适应的pH值范围为5～13，EV菌耐碱性极强，且在加有松树各部位成分的培养基上生长，菌株EV120806对松树培养基的适应性更强，未来有望将EV菌定殖在松树中做为共生菌，摄食入侵的松材线虫，从而使松树具有内源的防控因子防控松材线虫。

松毛虫(Dendrolimus spp.)是我国第一大森林害虫,其中马尾松毛虫(D. punctatus(Walker))占很大比例。由于苏云金芽孢杆菌(Bacillus thuringiensis Berliner,简称B.t)杀虫剂对松毛虫的危害具有良好的防治效果,因此在松毛虫灾害的治理中B.t杀虫剂倍受重视,并广为推广应用[1]。但在利用B.t杀虫剂防治越冬代的松毛虫时,发现其防治效果不佳,可能是当时气温较低所致。本研究就是针对不同温度下B.t杀虫剂对松毛虫的防治效果进行的,旨在为利用B.t杀虫剂防治越冬代松毛虫提供理论依据。

根据昆虫病原真菌毒素的分子量和结构分为低分子量毒素和高分子蛋白毒素两大类,根据结构将低分子量毒素分为环缩肽类、色素类、有机酸类和其它类,根据功能将大分子蛋白毒素分为酶类毒素和非酶类蛋白毒素。对白僵菌素、破坏素等多种昆虫病原真菌毒素的结构和性质进行了阐述。从昆虫病原真菌产毒素的适宜培养条件到毒素的分离纯化方法、毒素毒力的生物测定和毒素的致病机理等方面的研究进展情况进行了评述。

用不同浓度的HaNPV病毒感染3龄棉铃虫幼虫并分别收集不同处理的存活试虫进行室内传代饲养。结果表明,病毒对寄主昆虫不仅具有直接致死作用,而且对蛹质量和化蛹均有明显的影响,尤其是对亲代和子一代的寄主昆虫。同时,通过PCR技术,从子一代的幼虫和蛹中成功检测到该病毒,从而在分子水平上证明了病毒能够经过亲代传递到子代的种群中。这对病毒的高效利用和害虫的持续控制具有重要的理论和实践意义。

This paper reported the biological activities of five synthetic philanthotoxin analogs.Trials under laboratory condition indicated a certain level of activity on poisoning and inhibiting emergence rate.The LC50 of compound 1 on Ectropis obliqua was 8.987×103 mg·L-1.At the 8×103 mg·L-1 dosage of compound 1,the adjustment rate of pupation and emergence of E.obliqua was 52.44% and 89.81% respectively.The compound 2 had a certain effect on the larval and pupal weight.

利用PCR-RFLP鉴定体系和SDS-PAGE表达蛋白的分析方法,分析了来自我国不同森林立地带(寒温带、中温带、暖温带、北亚热带、中亚热带、南亚热带、高原亚热带、热带)自然保护区森林土壤中分离的72株苏云金芽孢杆菌的cry1、cry2、cry3、cry4、cry5、cry8、cry9、cry10、cry11、cry1I杀虫晶体蛋白基因类型,表达蛋白和杀虫活性的生物测定。研究表明:同时含有cry1、cry2、cry1I3类基因的有21株菌,6株菌含有cry1、cry2类基因,4株菌含有cry1和cry1I基因,只含有cry1基因的有1株,cry2基因的4株,36株菌不含所鉴定的10类基因。同时证明:绝大多数含有cry1基因的菌株表达了130kD蛋白,含有cry2基因的菌株表达了60kD的蛋白。对不同农、林害虫棉铃虫、杨扇舟蛾、舞毒蛾、马尾松毛虫、黄粉甲、榆兰叶甲、落叶松叶蜂等幼虫的杀虫活性进行了生物测定。进一步证明了苏云金芽孢杆菌cry基因,表达蛋白及杀虫活性三者的相关性。为生产和科研提供了生物治虫、抗虫育种新的苏云金芽孢杆菌cry基因资源。

根据茶尺蠖核型多角体病毒(EoNPV)多角体蛋白基因建立了PCR检测方法,在茶尺蠖子代卵和蛹内检测到EoNPV存在,灵敏度达到1 fg,此方法可用于田间施用EoNPV后茶尺蠖种群带毒检测。PCR方法由于操作简单、检测灵敏度高、特异性好、重复性好等优点,值得应用。利用EoNPV感染茶尺蠖2龄幼虫,幼虫对EoNPV的敏感性很高,测得LC50为2. 2 ×104 P IB·mL-l , EoNPV感染浓度越高,则幼虫死亡越快,幼虫死亡时间与感染浓度呈正相关。

The combinations of Hyphantria cunea NPV (HcNPV) and three different fluorescent brighteners BA,VBL, CBS-X were added to the artificial diet and the influence of synergists in concentration 25 ℃ and 14L /10D( h). The results showed that the fluorescent brightenersBA and VBL had higher insecticidal activity, CBS-X had notsignificant synergistic effect.

Hyphantria cuea nucleopolyhedrovirus (HcNPV) and several other nucleopolyhedrosis was extracted bydifferential centrifugation, and purified with sucrose gradient centrifugation method. Polyhedrin was gotten andanalysed by SDS - PAGE. It indicated thatmost of those p roteins had 32 KD p rotein band, and with 64 KD p rotein,and might be the main p rotein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virusp rotein in 18 KD department had a specific band.